HIVe Home
Posted: 31 Oct 2011
Analytical characterization of monoclonal antibodies Internship-Leiden, The Netherlands
Apply Here
B cell immunology and antibodies

Crucell is a global biopharmaceutical company dedicated to bringing meaningful innovation to global health. We do this by discovering, developing, manufacturing and marketing products that combat major threats to the health of people worldwide. Our specialty is fighting infectious diseases—a growing healthcare challenge.

Innovation is the drive force behind our strong research & development (R&D) pipeline, with promising products in pre-clinical and clinical development. Crucell product candidates include flu-mAb, and antibody product effective against a broad range of influenza virus strains, tuberculosis and malaria vaccines, as well as a rabies monoclonal antibody combination-all produced on our unique PER.C6® human-cell technology.

Currently we are combating twelve major infectious diseases with our range of marketed vaccines in the paediatric, travel & endemic and respiratory fields. 

The combination of our innovative technologies and creative minds drives Crucell’s success, which we measure in human and business terms. 

Johnson & Johnson completed the tender offer for Crucell N.V. (Crucell) on 22nd February 2011 and declared the offer unconditional. As a result, Crucell will now operate as the center for vaccines within the Johnson & Johnson pharmaceuticals group.

Headquartered in Leiden, the Netherlands, Crucell currently employs 1400 people and has offices in China, Indonesia, Italy, Korea, Malaysia, Spain, Sweden, Switzerland, the UK the USA and Vietnam.


Innovation and Discovery Laboratories (I&DL)
The suitable candidate will be working in the Protein Sciences group within the Pre-Clinical and Clinical Research group of the I&DL department. The Protein Sciences group is involved in the generation of stable cell lines of PER.C6® cells expressing monoclonal antibodies against infectious diseases and the manufacturing and characterization (USP, DSP and Analytical Assays) of material for proof of concept studies in animal models.

Job Description:

The activities in our group are focused on the analytical characterization of monoclonal antibodies and the implementation and development of analytical assays. This internship will focus on three major issues: 

  • Applying analytical assays to comprehensively characterize mAbs and identify process dependent modifications in their biophysical properties ;
  • Formulation and stability studies to identify the optimal formulation for the mAb drug candidate;
  • Addressing limitations in analytical assays – method development.
  • Analytical assays include Chromatography methods (SEC, Reversed Phase, etc), Asymmetric Flow Field-Flow Fractionation, Multi angle and Dynamic Light Scattering, Differential Scanning Calorimetry, Capillary Electrophoresis, Circular Dichroism, Fluorescence and Mass Spectrometry.

Minimum Qualifications:

Master/bachelor student in biophysical, (bio)chemical, biomedical, pharmaceutical or similar field
Experience with proteins and/or analytical assays
Independent and pragmatic 
Sociable character
40Hrs/week for 6 months or more


We are looking for a motivated individual who enjoys working in a multidisciplinary team. You express yourself well in English, both verbal and in writing. You are flexible in work and behavior. You enjoy being part of a team, but you are also capable of working independently.

As part of Crucell's recruitment policy, a pre-employment screening will take place during the recruitment process.


For more information:

Adrian Apetri (Senior Scientist Analytics), Tel. +31 71 5197054

To send an application, use the  "Apply here" button above or send your short letter of motivation and CV to:
Human Resources
P.O. Box 2048
2301 CA Leiden